BCR 2.0 0
Switchable Antigen-Sensing System (S.A.S.S.)
| Center |
|---|
To do's:
- Midiprep (from freezer)
- S9TEV
- B15T15
- Golden Gate
- B9T9
- B15T9
- LR
- hEF1a-CuO:Gmab H
- hEF1a-CuO:Gmab L
- hEF1a-CuO:CD79A
- hEF1a-CuO:CD79B
- hEF1a-CuO:Lyn
- Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Experimental Progress:
Cloning Progress
Parts Tree
Cloning Strategy
Experiments
Linker Optimization
Antibody Choices
Useful Links:
Protocols
Immediate To Do's:
| Task | Claimed by | Date Completed | Other Notes |
|---|---|---|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 |
|
| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| AarI golden gate of CD79A construct | LA | Apr 30 | |
| Take out CD79A golden gate plates | LA | May 2 | |
Make LB | CR | May 4 | |
CD79A golden gate liquid cultures | LA | May 4 | Cultures in at 6pm - need to be taken out by 10/11 am May 5 in 215 shaking incubator |
| Take out CD79A golden gate liquid cultures | LA | May 5 | In 4C |
CD79A golden gate minipreps | LA | May 6 | In yellow rack in 4C - need to be nanodropped |
CD79A golden gate restriction verification | CR | May 6 | |
| Redesign CD79B reverse PCR primer | |||
| CD79A linker golden gates | |||
- task dependent on above task
## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)
Non-Lab To Do's
- Bug Brian about ordering antibody testing supplies
Higher Level To Do's:
- Finish Discussing Figures for Experiment listDecide on other antigen-variable regions we want to test Syk-Lyn experiment design
- Receptor dilution experiment design
- Other experimental planning
- Is gantenerumab proprietary and can we actually publish with it?