TRANSFECTION PLANS
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| Name | Location | Concentration | | pEXPR: Hef1aMAV1212-hEF1a-eBFP2 | Our -20 | TC Room | Midi preppedCurrently being propagated | | pEXPR: Hef1ahEF1a-L7ae | Our -20 | | TC Room | Midi prepped | | pEXPR: CAG-2xKturn-eGFP (wt) | TC Room | Midi prepped | Currently being propagated | | pEXPR: Hef1aCAG-2x Kturn-eYFP | Nelson's lab bench | exKturn-eGFP (mut) | TC Room | Midi preppedWill be propagated when obtained |
EXPERIMENTS - Hef1a hEF1a eBFP2 & Hef1a hEF1a 2x Kturn eYFP (positive control)
- Should be yellow and blue
- Hef1a hEF1a eBFP2 & Hef1a hEF1a 2x Kturn eYFP & Hef1ahEF1a-L7ae (negative control)
- Should be blue (minimal yellow)
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| Name | Location | Concentration | | pEXPR: Hef1ahEF1a-eBFP2 | Our -20 | TC Room | Midi preppedCurrently being propagated | | pEXPR: Hef1ahEF1a-Gal4Vp16 | With Jin | Gal4VP16 | Our -20 | Verified, colony being picked for midi prepGetting in contact with Jin | | pEXPR: Hef1ahEF1a-TAL14 | With Brian | Our -20 | Being midi preppedpENTR will be LR'd with Hef1a when obtained | | pEXPR: 5xUAS/4xTAL14bsTAL14-eYFPmKate | With Samira | TC Room | Midi preppedUAS needs to be added somehow |
EXPERIMENTS: - Hef1ahEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
- negative control (minimal yellow)
- Hef1ahEF1a-eBFP2 & Hef1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
- positive control (hopefully linear BFP/YFP)
- Hef1ahEF1a-eBFP2 & Hef1a5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & Hef1ahEF1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
- Should be blue (minimal yellow)
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| name | Repressor co-transfection experimental designs |
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n-WELL PLATE PROPORTIONS:
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