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TRANSFECTION PLANS

Expand
titleL7ae Repression
NameLocationConcentration
MAV1212-hEF1a-eBFP2TC RoomMidi prepped
pEXPR:hEF1a-L7aeTC RoomMidi prepped
pEXPR: CAG-2xKturn-eGFP (wt)TC RoomMidi prepped
pEXPR: CAG-exKturn-eGFP (mut)TC RoomMidi prepped

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a
  •  pEXPR: Hef1a-eBFP2 - in freezer
  •  pEXPR: Hef1a-L7ae - in freezer
  •  pEXPR: Hef1a-2x Kturn-eYFP - with Nelson

EXPERIMENTS

  1. Hef1a eBFP2 & Hef1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. Hef1a hEF1a eBFP2 & Hef1a hEF1a 2x Kturn eYFP & Hef1ahEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

Expand
titleTAL14 Repression
NameLocationConcentration
 
pEXPR:
Hef1a
hEF1a-eBFP2
- in freezer
 
TC RoomMidi prepped
pEXPR:
Hef1a
hEF1a-
Gal4Vp16 - possibly with Jin? (get in contact with him)
Gal4VP16Our -20Verified, colony being picked for midi prep
pEXPR: hEF1a-TAL14Our -20Being midi prepped
pEXPR: TAL14-mKateTC RoomMidi prepped

 

EXPERIMENTS:

  1. hEF1a
  •  pEXPR: Hef1a-TAL14 - Brian has, in pENTRY form
  •  pEXPR: 5xUAS/4xTAL14bs-eYFP - getting from Samira (but missing UAS)

EXPERIMENTS:

  1. Hef1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. Hef1ahEF1a-eBFP2 & Hef1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
    1. positive control (hopefully linear BFP/YFP)
  3. Hef1ahEF1a-eBFP2 & Hef1a5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & Hef1ahEF1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
    1. Should be blue (minimal yellow)

 

n-WELL PLATE PROPORTIONS:

...

Gliffy Diagram
nameRepressor co-transfection experimental designs

 

 

 

...