hef1a - IgM
hef1a - IgK
hef1a - CD79A
...
| Gene | Promoter | Expression Vector | Why? | Location/Status |
|---|---|---|---|---|
| GmabH | hEF1a | pZDonor 1-GTW-2 | 1st in Gibson | designed |
| GmabL | hEF1a | pZDonor 2-GTW-3 | 2nd in Gibson | designed |
| CD79A | hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson? | designed |
...
| CD79A-TCS-Gal4VP16 |
...
hef1a - CD79B
| hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson? | designed | |
| CD79B | hEF1a | pZDonor 4-GTW-5 | 4th in Gibson? | designed |
| CD79B-TCS-Gal4VP16 |
...
hef1a - Lyn
hef1a - Syk?
...
| hEF1a | pZDonor 4-GTW-5 | 4th in Gibson? | designed | |
| Lyn | hEF1a | pZDonor 1-GTW-2 | designed |
| Lyn-mKate |
...
| Tet | pZDonor 1-GTW-2 | designed | |
| Syk-eYFP |
...
| Tet | pZDonor 2-GTW-3 | designed | |
| Syk-TEVp |
TETr - eBFP
| Tet | pZDonor 2-GTW-3 | designed | ||
| eBFP | Tet | pZDonor 1-GTW-2 (already in 1-2) | Control fluorescence for pTet promoter | in someone's freezer |
| mKate | GAL4/UAS | pZDonor 1-GTW-2 (already in 1-2) | Test activation by transcription factor (Gal4VP16) | in someone's freezer |
| rtTA | hEF1a | pZDonor 1-GTW-2 (already in 1-2) | Required for Tet promoters | in someone's freezer |
Do we want hEF1a - Syk?
(gal4 target) - mKate