Page History

...

Cloak

id	7-16

Transformed LR products

Digested MAV1212-Hef1a-L7ae with BsmBI in 3.1 Buffer

14-7-17

Toggle Cloak

id	7-17

Cloak

id	7-17

Miniprep and Midiprep

Transformations

MAV1212-Hef1a-Gal4VP16

MAV1212-TRE-Gal4VP16

MAV1212-TRE-L7ae

MAV1212-Hef1a-eBFP2-miR181

MAV1212-Hef1a-eBFP2-miR144

MAV1212-Hef1a-L7Ae-miR146

MAV1212-Hef1a-L7Ae-miR7

MAV1212-Hef1a-L7Ae-miR125

MAV1212-Hef1a-L7Ae-miR30

14-7-18

Toggle Cloak

id	7-18

Cloak

id	7-18

DNA	Con (ng/µL)	Enzyme	Buffer	Vol DNA	Mass DNA	Vol Enzyme	Vol Buffer	Vol Water	Vol Total
Ladder
MAV1212-Hef1a-Gal4VP16	305.5	BsaI	3.1	2.45	750	1	2.00	14.55	20
MAV1212-Hef1a-Gal4VP16 control	305.5			2.45	750			17.55	20
MAV1212-TRE-Gal4VP16	74.3	BsaI	3.1	10.09	750	1	2	6.91	20
MAV1212-TRE-Gal4VP16 control	74.3			10.09	750			9.91	20
MAV1212-TRE-L7ae	58.7	BsaI	3.1	12.78	750	1	2	4.22	20
MAV1212-TRE-L7ae control	58.7			12.78	750			7.22	20
MAV1212-Hef1a-eBFP2-miR181	118.6	BsaI	3.1	6.32	750	1	2	10.68	20
MAV1212-Hef1a-eBFP2-miR181 control	118.6			6.32	750			13.68	20
MAV1212-Hef1a-eBFP2-miR144	49.9	BsaI	3.1	15.03	750	1	2	1.970	20
MAV1212-Hef1a-eBFP2-miR144 control	49.9			15.03	750			4.97	20
Ladder

14-7-19

Toggle Cloak

id	7-19

Cloak

id	7-19

DNA	Con (ng/µL)	Enzyme	Buffer	Vol DNA	Mass DNA	Vol Enzyme	Vol Buffer	Vol Water	Vol Total
Ladder
MAV1212-TRE-Gal4VP16	74.3	BsaI	3.1	6.73	500	1	2	10.27	20
MAV1212-TRE-Gal4VP16 control	74.3			6.73	500			13.27	20
MAV1212-TRE-L7ae	58.7	BsaI	3.1	8.52	500	1	2	8.48	20
MAV1212-TRE-L7ae control	58.7			8.52	500			11.48	20
MAV1212-Hef1a-eBFP2-miR181	118.6	BsaI	3.1	4.22	500	1	2	12.78	20
MAV1212-Hef1a-eBFP2-miR181 control	118.6			4.22	500			15.78	20
MAV1212-Hef1a-eBFP2-miR144	632.6	BsaI	3.1	1.581	1000	1	2	15.42	20
MAV1212-Hef1a-eBFP2-miR144 control	632.6			1.581	1000			18.42	20
MAV1212-Hef1a-eBFP2	50.3	BsaI	3.1	9.94	500	1	2	7.06	20
MAV1212-Hef1a-eBFP2 control	50.3			9.94	500			10.06	20
Ladder

14-7-21

Toggle Cloak

id	7-21

Cloak

id	7-21

Why why why why why

Found out that we have no low sensors.

14-7-22

Toggle Cloak

id	7-22

Cloak

id	7-22

MAV1212-TRE-L7ae

Incomprehensible bands, recommend re-LR.

14-7-23

Toggle Cloak

id	7-23

Cloak

id	7-23

weeeeee!

14-7-24

Toggle Cloak

id	7-24

Cloak

id	7-24

MAV1212-Hef1a-TAL14

EcoRI

14-7-25

Toggle Cloak

id	7-25

Cloak

id	7-25

Golden Gate
Combined Low Sensor

14-7-26

Toggle Cloak

id	7-26

Cloak

id	7-26

Midi Prep
MAV1212-Hef1a-GAL4VP16 MAV1212-TRE-GAL4VP16 Single Low Sensors

Transformation
MAV1212-Hef1a-eBFP2-miR144-181c

14-7-28

Toggle Cloak

id	7-28

Cloak

id	7-28

Child pages

Versions Compared

Old Version 2

New Version Current

Key