...
- Nanodrop pENTR (was not useful)
- Transform pENTR into bacteria
- Incubate over night
- (5pm) Add Kan, incubate GGDonr liq culture (16hrs)
Saturday:
- Remove GGDonr liq culture from incubator (9am, Lyla)
- Make cell stock, store at -80 degrees
- Put rest of cell culture in 4 degrees
- Remove pENTR cultures from incubator (10:30 am30am, Shinjini)
- Pick cells from pENTR colonies, put in liquid culture, put in incubator at 12pm
- Pick cells from pENTR colonies, put in liquid culture, put in incubator at 5:30pm (16 hours)
...
Sunday:
- Take pENTR colonies from incubator. Make cell stock. Put in fridge and freezer respectively. (10 am). Did not work.
Monday:
- Make empty liquid cell culture to transform DNA into (~16hours)
- Make 5 femptomolal Miniprep GGDonr (is that a good idea?)
- Pick Cells again and incubate (at 6pm)
...
Tuesday:
- Miniprep pENTRs
- Make 5 femptomolar working solution
- LR (~ 16 hours min, 24 max)
- Miniprep GGDonr
- PCR (possibly)
June 9
Plan of Action
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