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Species

Strain

E.coli (background K12) --> BL1

HCB33 (TSS496): E. coli wild type
HCB1584: E. coli D(tar)
HCB268: E. coli tsr::Tn5-1a
TSS945: E. coli D(tsr)
UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer)
UU2162: D(tar tap) D(tsr) D(trg) D(aer)
TSS584: HCB33 / pVS129 (ecfp on pTrc99A)
TSS585: HCB33 / pVS132 (eyfp on pTrc99A)
TSS586: HCB33 / mCherry on pTrc99A

E. coli (background DH5a) --> BL1

TSS379
TSS342
TSS270
TSS712
TSS711
TSS943
TSS694
HCB1666

Salmonella (background LT2) --> BL2 (attenuated)

LT2 (TSS495): Salmonella LT2 wild type
LT2 (TSS497): Salmonella LT2wild type pSLT cured
TSS511: Salmonella LT2 D(cheV)
TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ
TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33
TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt)
TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc)
LT2 RecA: strain with the recA gene inactivated. kanamycin resistance is inserted in the mutated site.
LT2 recAo: strain with the SOS box mutated. Chloramphenicol resistance is inserted in the mutated site.
LT2 LexA(Ind-): strain with the lexA gene mutated. Chloramphenicol resistance is inserted in the mutated site.
LT2 CheY: strain with the cheY gene inactivated. Chloramphenicol resistance is inserted in the mutated site.
LT2 CheB: strain with the cheB gene inactivated. Chloramphenicol resistance is inserted in the mutated site.
TSS587: TSS497 / pVS129 (ecfp on pTrc99A)
TSS588: TSS497 / pVS132 (eyfp on pTrc99A)
TSS589: TSS497 / mCherry on pTrc99A

Salmonella (background Salmonella 14028) --> BL2

MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip

Melissa

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Escherichia coli (WT)

RP437 (from Linda Turner, Berg's Lab)

Melissa 

   

Name

Strain

Media to use

Location

Vibrio coralliilyticus

BAA-450 (directly from ATCC; used for ISME J 2014 paper)

2216 (or TCBS plates to select for Vibrios if contamination suspected)

 

 

 

 

 

 

 

Stocker 01

Vibrio coralliilyticus

P1 GFP (from Joe Pollock/David Bourne)

2216 (or TCBS plates to select for Vibrios if contamination suspected)Stocker 01
Vibrio coralliilyticusYB1 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)2216 (or TCBS plates to select for Vibrios if contamination suspected)Stocker 01
Vibrio coralliilyticusYB2 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)2216 (or TCBS plates to select for Vibrios if contamination suspected)Stocker 01
Vibrio coralliilyticusYB1 dsRed (from Orr Shapiro/Assaf Vardi)2216 + 50ug/ml KanStocker 01

Vibrio coralliilyticus

YB2 dsRed (from Orr Shapiro/Assaf Vardi)

2216 + 50ug/ml KanStocker 01
Vibrio coralliilyticusNon-motile (fla knockout) YB2 (from Dalit Meron-- sent to Rory while in Stocker lab)2216 + 100ug/ml Kan (though 50ug should be OK too-- they were originally isolated on 200ug/ml selective medium)Rory&Mel's box
Vibrio fortisisolated from healthy coral (non-pathogenic; from Rory Welsh/Becky Vega-Thurber)2216 (or TCBS plates to select for Vibrios if contamination suspected)Rory&Mel's box
Serratia marcescenscoral pathogen from Katie Patternson's lab via Rory/Becky2216 Rory&Mel's box
Bacteriovoraxisolated from healthy coral (preys on V.cor and V.fortis; from Rory Welsh/Becky Vega-Thurber)grows on lawn or culture of vibrio (coralliilyticus or fortis)Rory&Mel's box
Pseudovibrio sp.isolated from healthy coral by JB Raina. Produces the antimicrobial compound TDA2216 Stocker 01

Cherry

Ruegeria pomeroyi DSS-3 strain, in 2216 media, from Mary Ann Moran's lab (March 2014)

Yutaka

 

 

BOX2

 

 

A

1

Vibrio cycrotrophicus

1F111

hsp60 confirmed

A

2

Vibrio cycrotrophicus

1F175

 

A

3

Vibrio cycrotrophicus

1F273

hsp60 confirmed

A

4

Vibrio cycrotrophicus

1F289

hsp60 confirmed

A

5

Vibrio cycrotrophicus

1F53

hsp60 confirmed

A

6

Vibrio cycrotrophicus

1F97

hsp60 confirmed

A

7

Vibrio cycrotrophicus

FF160

 

A

8

Vibrio cycrotrophicus

FF274

 

A

9

Vibrio cycrotrophicus

FFA

 

B

1

 

 

 

B

2

Vibrio cycrotrophicus

ZF14

hsp60 confirmed

B

3

Vibrio cycrotrophicus

ZF205

 

B

4

Vibrio cycrotrophicus

ZF270

hsp60 confirmed

B

5

Vibrio cycrotrophicus

ZF28

 

B

6

Vibrio cycrotrophicus

ZF30

hsp60 confirmed

B

7

Vibrio cycrotrophicus

ZF65

hsp60 confirmed

B

8

Vibrio cycrotrophicus

ZF99

 

B

9

 

 

 

 

 

Box 01

Strain

B

1

Vibrio sp.

12B9 GFP

B

2

Vibrio sp.

12B9RFP

B

3

Vibrio cycrotrophicus

1F53 GFP

B

4

Vibrio cycrotrophicus

1F53 RFP

B

5

Vibrio cycrotrophicus

1F111 GFP

B

6

Vibrio cycrotrophicus

1F111 RFP

B

7

Vibrio cycrotrophicus

ZF270 GFP

B

8

Vibrio cycrotrophicus

ZF270 RFP

B

9

 

 

C

1

Vibrio crassostreae

13D11 GFP

C

2

Vibrio crassostreae

13D11 RFP

C

3

Vibrio sp.

ZF223 GFP

C

4

Vibrio sp.

ZF223 RFP

C

5

Vibrio cycrotrophicus

FF274 RFP

C

6

 

 

C

7

 

 

C

8

 

 

C

9

 

 

D

1

Vibrio cycrotrophicus

ZF65

D

2

Vibrio cycrotrophicus

ZF270

D

3

Vibrio ordalii

FS144

D

4

Vibrio ordalii

FF93

D

5

Vibrio ordalii

1S257

D

6

Vibrio ordalii

12B9

D

7

(Vibrio cycrotrophicus)

(1F273)

D

8

(Vibrio cycrotrophicus)

(1F175)

D

9

Vibrio cycrotrophicus

1F111

E

1

Vibrio cycrotrophicus

ZF14

E

2

(Vibrio cycrotrophicus)

(1F289)

E

3

(Vibrio cycrotrophicus)

(1F289)

E

4

V. alginolyticus

YM4

E

5

Vibrio cycrotrophicus

ZF30

E

6

Vibrio cycrotrophicus

ZF99

E

7

Vibrio cycrotrophicus

ZF205

E

8

Vibrio cycrotrophicus

ZF28

E

9

Vibrio cycrotrophicus

FF160

F

1

Vibrio cycrotrophicus

1F53

F

2

Vibrio cycrotrophicus

1F97

F

3

Vibrio cycrotrophicus

FF160

F

4

 

 

F

5

 

 

F

6

 

 

F

7

 

 

F

8

 

 

F

9

 

 

F

 

 

 

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