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Inducible and Constitutive Cascades

Syk-TEVp Cleavage

 

  •  B Cells
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  RPMI1640
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Anti IgM Green
  •  Secondary?
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
  •  TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Biotinylated aBeta
  •  Anti IgM Green
NumberNameParts/ReagentsStatus
1Membrane Localization of BCR 
Expand
titleParts/Reagents

Ongoing

2Beta Amyloid Binding of Gmab in BCR Complex 
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Biotinylated aBeta
  •  Streptavidin-RED

Ongoing

3Syk Western Blotexpand

Complete

titleParts/Reagents
  •  ?
4Membrane Localization of Lyn 

Waiting on Membrane Localization

54Membrane Localization of Lyn 
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  TRE: Lyn-mKate
  •  hEF1a: rtTA
  •  Dox
  •  Biotinylated aBeta
5Membrane Localization of Syk
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  hEF1a: Lyn
  •  TRE: Syk-eYFP
  •  hEF1a: rtTA
  •  Biotinylated aBeta
6Inactive TANGO Test
Expand
titleParts/Reagents
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
  •  TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Dox
7Active TANGO Test
Expand
titleParts/Reagents
SykWaiting on Membrane Localization
6Syk-TEV Cleavage

Ready

7Lyn-TEV Cleavage

Waiting on Parts

8Syk-TEV & Lyn CleavageWaiting on Parts

Template

Expand
titleTest 1
Expand
titleDescription

 

Expand
titlePurpose

 

Expand
titleParts Needed

 

Expand
titleSetup

Well 1

HEK293

Well 2

HEK293

Well 3

HEK293

Well 4

HEK293

Well 5 

HEK293

Well 6 

HEK293

Well 7

HEK293

Well 8 

HEK293

Well 9 

HEK293

Well 10 

HEK293

Well 11 

HEK293

Well 12 

HEK293

Well 13 

HEK293

Well 14 

HEK293

Well 15 

HEK293

Well 16 

HEK293

Well 17 

HEK293

Well 18 

HEK293

Well 19 

HEK293

Well 20

HEK293

Well 21 

HEK293

Well 22 

HEK293

Well 23 

HEK293

Well 24 

HEK293

Expand
titleExpected Results

 

Expand
titleProtocol

 

Expand
titleMicroscopy Data

 

Expand
titleCytometry Data

 

Expand
titleAnalysis