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Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment | Expected Results | Protocols | Parts | Completed? |
|---|---|---|---|---|
| Construct U6/miRNA | ||||
| Assay down-regulation of BACE1 by U6/miRNA | ||||
| Construct miRNA/BACE2 fusion (also including YFP) | ||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 | ||||
Appendix
Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment |
|---|
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2-YFP |
Purchases
| Item | Link |
|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 |
| BACE1 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 |
| Bace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 |
| Bace1 activity assay | http://www.anaspec.com/products/product.asp?id=57888 |
data sets