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LR Reaction:

Label

Gene

(fmol)

Promoter

(fmol)

Destinatioin

(fmol)

LR Clonase(uL)

Total Volume

JX 1

mKate

5

Het1a-LacO

5

1-2

10

1

4

JX 2

rtTA

5

Het1a

5

1-2

10

1

4

14-Mar-14

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Gibson Reaction Mix

Sample Y166

 

DNA

I-Sce-1

Buffer

Water

Total

Mass (μg)

1

 

 

 

 

Concentration (ng/μL)

316

 

 

 

 

Volume (μL)

3.16

1

2

13.84

20

Order

2

4

3

1

 

Created Gibson reactio mix. Reactants entered in volume and order noted in table.

14-Mar-16

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