BCR 2.0
Cloning Progress
Parts Tree
Cloning Strategy
Planned Experiments
Linker Optimization
Antibody Choices
Things to do:
| Task | Claimed by | Date Completed | Other Notes |
|---|---|---|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 |
|
| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| CD79s PCR with new primers | |||
| Gel verification of CD79 PCRs with new primers | |||
| AarI golden gates |
To Do:
- Redesign CD79B PCR primers
- AarI Golden GateCD79A-pTet:RFP-Gal4VP16
- Gel Extraction of Q1-CD79A-Q3
- Column Purification of pTet-RFP and Gal4VP16 PCR products
- Establish Cryostocks of GmabH, GmabL, Syk-Tev, and Lyn-Tev pEXPRs and dilute DNA to 200 ng/uL
0
Switchable Antigen-Sensing System (S.A.S.S.)
| Center |
|---|
To do's:
- Midiprep (from freezer)
- S9TEV
- B15T15
- Golden Gate
- B9T9
- B15T9
- LR
- hEF1a-CuO:Gmab H
- hEF1a-CuO:Gmab L
- hEF1a-CuO:CD79A
- hEF1a-CuO:CD79B
- hEF1a-CuO:Lyn
- Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)
Non-Lab To Do's
- Bug Brian about ordering antibody testing supplies
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?
Useful Links