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Cloak
id3/7

LR Reaction:

Label

Gene

(fmol)

Promoter

(fmol)

Destinatioin

(fmol)

LR Clonase(uL)

Total Volume

JX 1

mKate

5

Het1a-LacO

5

1-2

10

1

4

JX 2

rtTA

5

Het1a

5

1-2

10

1

4

14-Mar-14

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id3/14

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id3/14

Gibson Reaction Mix

Sample Y166

 

DNA

I-Sce-1

Buffer

Water

Total

Mass (μg)

1

 

 

 

 

Concentration (ng/μL)

316

 

 

 

 

Volume (μL)

3.16

1

2

13.84

20

Order

2

4

3

1

 

...

 

...

DNA

...

I-Sce-1

...

Buffer

...

Water

...

Total

Created Gibson reactio mix. Reactants entered in volume and order noted in table.

14-Mar-16

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Gibson Reaction Mix for All Samples

Mass (μg)

Enzyme (μL)

Buffer (μL)

Total (μL)

 

1

1

2

20

 

 

 

...

Mass (μg)

...

 

 

 

 

...

Con (ng/μL)

Vol DNA (μL)

Vol H2O (μL)

...

316

...

 

...

 

...

 

...

 

...

Volume (μL)

...

3.16

...

1

...

2

...

13.84

...

20

 

1.1

151

6.62

10.38

CMU-Nde1

1.2

267

3.75

13.25

 

1.3

141

7.09

9.91

 

2.1

652

1.53

15.47

TRE-t-Sta1

2.2

263

3.80

13.20

 

2.3

212

4.72

12.28

 

4.1

149

6.71

10.29

TRE-t-Sta1

4.2

415

2.41

14.59

 

4.3

583

1.72

15.28

 

6.1

353

2.83

14.17

TRE-t-Sta1

6.2

593

1.69

15.31

 

6.3

361

2.77

14.23

 

7.1

448

2.23

14.77

Het1a-Xhol

7.2

237

4.22

12.78

 

7.3

186

5.38

11.62

 

8.1

246

4.07

12.93

Het1a-Lac-BamH1

8.2

182

5.49

11.51

 

8.3

871

1.15

15.85

 

9.1

410

2.44

14.56

TRE-t-Sta1

9.2

214

4.67

12.33

 

9.3

185

5.41

11.59

 

10.1

285

3.51

13.49

CMU-Nde1

10.2

547

1.83

15.17

 

10.3

388

2.58

14.42

 

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Gel Figure for Previous Samples:

...

Order

...

2

...

4

...

3

...

1

...