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We are currently varying two parameters of the system in order to find where the system operates best i.e. has the biggest difference in output between the +aBeta and -aBeta. The parameters are the amount of Receptor-TCS-Gal4VP16 construct transfected (since we have seen that a large amount of transfection places a strain on the cell) and how much TEVp-Cofilin is expressed.

Expand

title	Parameter Selection

Gliffy Diagram

name	Tuning

LilrB2

Expand

title	LilrB2 Trial 1 Transfection Plan

Trial 1 Transfection

Well 1

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

Well 2

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well 3

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

2000 nM Dox

Well 4

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

200 nM Dox

500 nM aBeta

Well 5

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

Well 6

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well 7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well 8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well 9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well 10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

500 nM aBeta

Well 11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well 12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

500 nM aBeta

Well 13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

Well 14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

500 nM aBeta

Well 15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

Well 16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Well 17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well 18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

500 nM aBeta

Well 19

HEK 293

Well 20

hEF1a:tagBFP 200ng

hEF1a:LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 21

hEF1a:tagBFP 200ng

hEF1a:LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 22

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 23

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Well 24

PirB

Expand

title	PirB LilrB2 Trial 1 2 Transfection Plan

Trial 1 Transfection

Well 2

PirB 200nghEF1a:

Dummy 200ng

Well 3

PirB 200nghEF1a:

Well 4

tagBFP 200nghEF1a:PirBG4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 5

PirB-TCS-G4VP16 hEF1artTA 200ng

500 nM aBeta

11PirB 625 19375 1 12PirB 625 19375 500 nM aBeta 13PirB 50ng150 10 14PirB 50ng150 10

500 nM aBeta

15PirB 50ng150 1000 16PirB 50ng150 1000 500 nM aBeta 17PirB 625 19375 2000 18PirB 625 19375 2000 500 nM aBeta 22Gal4UAS:mKate 200ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Well 23

hEF1a:tagBFP PirB-TCS-Gal4VP16 12.5 ng

Well 1

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25ng

Dummy 193.75 ng

0 nM Dox

Well 2

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-

Gal4VP16 6.25ng

Dummy 193.75 ng

0 nM Dox

500 nM aBeta

Well 3

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:

TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25ng

Dummy

Well 6

193.75 ng

1 nM Dox

Well 4

Well 7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng6.25ng

Dummy 100 193.75 ng

1 nM Dox

500 nM aBeta

Well

8

5

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng0.78 ng

Dummy 100 199.22 ng

1 0 nM Dox

500 nM aBeta

Well

9

6

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng0.78 ng

Dummy 100 199.22 ng

2000 0 nM Dox

500 nM aBeta

Well

10

7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng3.13ng

Dummy 100 196.87 ng

200 0 nM Dox

500 nM aBeta

Well

8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

3.

13ng

Dummy

196.

87 ng

0 nM Dox

500 nM aBeta

Well

9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

3.

13ng

Dummy

196.

87 ng

1 nM Dox

Well

10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

3.13ng

Dummy

196.87 ng

1 nM Dox

500 nM aBeta

Well

11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

0.78 ng

Dummy

199.22 ng

1 nM Dox

Well

12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

0.78 ng

Dummy

199.22 ng

1 nM Dox

500 nM aBeta

Well

13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

1.56ng

Dummy

198.44 ng

0 nM Dox

Well

14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

1.

56ng

Dummy

198.

44 ng

0 nM Dox

500 nM aBeta

Well

15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16

1.

56ng

Dummy

198.

44 ng

1 nM Dox

Well

19

16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 12.5 56ng

Dummy 187198.5 44 ng

10 1 nM Dox

500 nM aBeta

Well

20

17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 1212.5 ng

Dummy 187.5ng

10 1 nM Dox

500 nM aBeta

Well

21

18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 1212.5 ng

Dummy 187.5ng

1000 1 nM Dox

500 nM aBeta

Well 19

HEK 293

Well

20

hEF1a:tagBFP 200ng

hEF1a:LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 21

tagBFP 200ng

hEF1a:LilrB2

200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 22

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 12.5ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 387.5ng

Well 23

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 12.5ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 387.5ng

500 nM aBeta

Well 24

PirB

Expand

title	PirB Trial 1 Transfection Plan

Trial 1 Transfection

Well 1

Well 2

hEF1a:tagBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 3

hEF1a:tagBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 4

hEF1a:tagBFP 200ng

hEF1a:PirB-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 5

hEF1a:tagBFP 200ng

hEF1a:PirB-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Well 6

Well 7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

Well 8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well 9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

2000 nM Dox

Well 10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

200 nM Dox

500 nM aBeta

Well 11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

Well 12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well 13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well 14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well 15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well 16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

500 nM aBeta

Well 17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well 18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

500 nM aBeta

Well 19

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

Well 20

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

500 nM aBeta

Well 21

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

Well 22

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Well 23

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well 24

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

500 nM aBeta

Expand

title	PirB Trial 2 Transfection Plan

Well 1

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

Well 2

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well 3

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

2000 nM Dox

Well 4

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

200 nM Dox

500 nM aBeta

Well 5

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

Well 6

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well 7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well 8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well 9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well 10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

500 nM aBeta

Well 11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well 12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

500 nM aBeta

Well 13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

Well 14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

500 nM aBeta

Well 15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

Well 16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Well 17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well 18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

500 nM aBeta

Well 19

Well 20

hEF1a:tagBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 21

hEF1a:tagBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 22

hEF1a:tagBFP 200ng

hEF1a:PirB-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 23

hEF1a:tagBFP 200ng

hEF1a:PirB-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Well 24

RESULTS

LilrB2

Expand

title	LilrB2 Trial 1

Controls

Well 19

HEK 293

Well 20

hEF1a:tagBFP 200ng

hEF1a:LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 21

hEF1a:tagBFP 200ng

hEF1a:LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 22

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 23

hEF1a:tagBFP 200ng

hEF1a:LilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

Image Added

100 ng LilrB2

Well 1

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

Well 2

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well 3

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

2000 nM Dox

Well 4

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

200 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

50 ng LilrB2

Well 7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well 8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well 9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well 10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

25 ng LilrB2

Well 17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well 18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

500 nM aBeta

Image Added

Image Added

12.5 ng LilrB2

Well 13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

Well 14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

500 nM aBeta

Well 15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

Well 16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

hEF1a:PirB-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well 24

PirB 175ng

100 nM Dox

500 nM aBeta

6.25 ng LilrB2

Well 5

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

Well 6

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well 11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:LilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well 12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:

LilrB2-TCS-Gal4VP16 6.25 ng

Dummy

193.75 ng

2000 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

PirB

Expand

title	PirB Trial 2 Transfection Plan 1

Gal4UAS:mKate

Dummy 100 ng

1 nM Dox

Well 2

hEF1a:tagBFP PirB-TCS-Gal4VP16 100nghEF1a:PirB-TCS-Gal4VP16 100ng

Controls

Well 1

Well 2

hEF1a:tagBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well 3

tagBFP 200ng

hEF1a:PirB

200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well 4

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well 3

hEF1a:tagBFP 200ng

hEF1a:rtTAPirB-TCS-G4VP16 200ng

TREhEF1a:TEVp-Cofilin rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 5

hEF1a:tagBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 100ngG4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

Image Added

5 6.25 ng 193.75 6 6.25 ng 193.75 1 500 nM aBeta 7 50ng150 10

100 ng PirB

Well 7

Dummy 100 ng

2000 nM Dox

Well 4

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 100ng

Dummy 100 ng

200 1 nM Dox

500 nM aBeta

Well

8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

100ng

Dummy

100 ng

1 nM Dox

500 nM aBeta

Well

9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

100ng

Dummy

100 ng

2000 nM Dox

Well

10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

100ng

Dummy

100 ng

200 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

Well 8

500 nM aBeta 91000 10500 nM aBeta 11 6.25 ng 193.75 2000

50 ng PirB

Well 13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well

14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well

15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well

16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

50ng

Dummy

150 ng

1000 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

12 6. 193.75 2000 500 nM aBeta

25 ng PirB

Well

23

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

25 ng

Dummy

175ng

100 nM Dox

Well

13

24

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 25 ng

Dummy 187.5 175ng

10 100 nM Dox

500 nM aBeta

Image Added

Image Added

17 25 175100

12.5 ng PirB

Well

14

19

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox500 nM aBeta

Well

15

20

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 10 nM Dox

500 nM aBeta

Well

16

21

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox500 nM aBeta

Well

22

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16

12.5 ng

Dummy

187.5 ng

1000 nM Dox

500 nM aBeta

Image Added

Image Added

Image Added

Image Added

Image Added

Image Added

Well 18

175ng

100 nM Dox

500 nM aBeta

Well 20

PirB 200nghEF1a:

Dummy 200ng

Well 21

PirB 200nghEF1a:

Well 22

hEF1a:tagBFP 200ngG4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well 23

PirB-TCS-G4VP16 hEF1artTADummy 200ng

6.25 ng PirB

Well 11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy

193.75 ng

1 nM Dox

Well 19

Well 12

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well 17

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate

200ng

hEF1a:PirB-TCS-

Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well 18

hEF1a:tagBFP 200ng

rtTA 200ng

TRE:

TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirB-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

500 nM aBeta

Well 24

RESULTS


Image Added	Image Added	Image Added	Image Added
Image Added	Image Added	Image Added	Image Added

Expand

title	PirB Trial 12

Controls

Well

1

19

HEK 293

Well

2

20

hEF1a:tagBFP 200ng

hEF1a:PirB LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Dummy 200ng

Well

3

21

hEF1a:tagBFP 200ng

hEF1a:PirB LilrB2 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

Well

4

22

hEF1a:tagBFP 200ng

hEF1a:PirBLilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

Well

5

23

hEF1a:tagBFP 200ng

hEF1a:PirBLilrB2-TCS-G4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate 200ng

Dummy 200ng

500 nM aBeta

Image Removed

Image Removed

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Image Removed

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100 ng PirBLilrB2

Well

7

1

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

Well

8

2

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

1 nM Dox

500 nM aBeta

Well

9

3

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

2000 nM Dox

Well

10

4

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 100ng

Dummy 100 ng

200 nM Dox

500 nM aBeta

Image Removed

Image Added

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Image Added

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Image Added

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Image Added

50 ng PirBLilrB2

Well

13

7

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

Well

14

8

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

10 nM Dox

500 nM aBeta

Well

15

9

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

Well

16

10

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 50ng

Dummy 150 ng

1000 nM Dox

500 nM aBeta

Image Removed

Image Added

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Image Added

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Image Added

Image Removed

Image Added

25 ng PirBLilrB2

Well

23

17

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

Well

24

18

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 25 ng

Dummy 175ng

100 nM Dox

500 nM aBeta

Image Removed

Image Added

Image Removed

Image Added

12.5 ng PirBLilrB2

Well

19

13

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

Well

20

14

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

10 nM Dox

500 nM aBeta

Well

21

15

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

Well

22

16

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 12.5 ng

Dummy 187.5 ng

1000 nM Dox

500 nM aBeta

Image Removed

Image Added

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Image Added

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6.25 ng PirBLilrB2

Well

11

5

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

Well

12

6

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

1 nM Dox

500 nM aBeta

Well

17

11

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

Well

18

12

hEF1a:tagBFP 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate 200ng

hEF1a:PirBLilrB2-TCS-Gal4VP16 6.25 ng

Dummy 193.75 ng

2000 nM Dox

500 nM aBeta

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