Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree
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miRNA Sensors (Theory)
- Propose experiments to test L7ae and Tau14/21
- Find restriction enzymes to digest original EF
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miRNA Sensors (Lab Work)
- Miniprep original EF
- PCR mRFP out of pL1F_1_S6_S7
- PCR Purify mRFP
- Run the gel for mRFP
- Re Golden Gate EF and GH
- Digest mRFP cassette
- Re ligate mRFP cassette
- Transform mRFP cassette
- Retransform EF and GH
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NOTES
There were no colonies to pick from EF and GH --> re transform