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MINI-PREP:

  • Prepare culture in a 15mL, round bottom tube.
  • Add

...

  • 3-

...

  • 5mL LB
  • Add 3-

...

  • 5uL (respectively) of antibiotic (Ampicillin, Kanamycin, etc.)
  • Pick colony using a pipette tip. Eject tip into tube (tip should remain in tube).


MIDI-PREP:

  • Prepare culture in a 500mL Erlenmeyer flask.
  • Add 50mL LB
  • Add 50uL of antibiotic (Ampicillin, Kanamycin, etc.)
  • Pick colony using a pipette tip. Eject tip into tube (tip should remain in tube).