Page History

TRELacIBufferEnzyme1.5

	hEF1a:CD79AeYFPhEF1a	TRE:CD79BeYFP	hEF1a:Lyn	hEF1a:GMabHTRE:mKate	hEF1a:GMabLhEF1a:CD79A-TCSRFP-G4 SDM #1	hEF1a:CD79BCD79A-TCSRFP-G4 TRE:Syk-15-TEVpSDM #2	hEF1a:rtTACymR	hEF1a:Gal4VP16hEF1a-LacOCuO:mKate	CMV5-CuO:mKate	hEF1a:	TRE:Gal4VP16	pENTR hEF1a-LacO	pENTR LacI	TRE:Syk	CD79A-3T3-G4 1,2,3,4	hEF1a:CD79A-3T9-G4 1,2	hEF1a:CD79A-3T15-G4 1,2,3,4
Number	1 - maybe, top band a little high	2 - success	3 - maybe, top band a little low	4 - can't tell because bottom band is hard to distinguish	5 - same as 4, plus it's more faint	Number	1	2	3	4	5	6	7	8	9 - maybe, there are also faint bands around 0.7kb and 2.0kb though 10 - too faint to tell 11 - fail, but extra bands at 4kb and at low kbs 12 - fail, but extra bands at 4kb and at low kbs	13 - fail, but extra band at 4kb 14 - fail, but extra band at 4kb	15 - fail, but extra band at 4kb 16 - fail, but extra band at 4kb 17 - fail, but extra band at 4kb 18 - fail, but extra band at 4kb
Enzyme	Enzyme	ApaI Cuts in hEF1a, CD79A & backbone	ApaI Cuts in hEF1a, and backbone	ApaI, NcoI Cuts in Lyn, hEF1a and backbone	BglII Cuts in GmabH, hEF1a and backbone	KpnI Cuts in GmabL, hEF1a, and backbone	PvuII Cuts in CD79A, TCS and backbone	PvuII Cuts in CD79B, TCS and backbone	PvuII Cuts in syk, tevp and backbone	ApaI Cuts in hEF1a, rtTA3 and backbone	XhoI Cuts in hEF1a, Gal4VP16 and backbone	NheI	PvuII	ApaI	XhoI	ApaI cuts in hEF1a-LacO	ApaI cuts in LacI	PvuII cuts in syk
Virtual gel	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed	Image Removed
Concentration (ng/uL)	1110.3	1420	1314.4	983.5	1359.0	1086.1	487.2	1161.9	706.4	441.9	741.0	483.1	420.6	1265.3	229.8	196.7	321.8
BlpI, XbaI	StuI	BsaI	BsaI				PvuII	PvuII	PvuII
Virtual gel	Image Added	Image Added	Image Added	Failure mode Image Added Success bands 5 kb 3.8 kb 0.85 kb	Failure mode Image Added Success bands 5 kb 3.8 kb 0.85 kb				Image Added	Image Added	Image Added
Concentration (ng/uL)
Buffer
Enzyme
DNA volume
H2O volume												0.45032874	0.352112676	0.3804017	0.508388409	0.367917586	0.460362766	1.026272578	0.430329632	0.70781427	1.13147771	0.674763833	1.03498241	1.18877794	0.395163202	2.175805048	2.54194204	1.55376	H2O volume	11.54967126	11.64788732	11.619598	11.49161159	11.63208241	11.53963723	10.97372742	11.56967037	11.2921857	10.86852229	11.32523617	10.9650176	10.8112221	11.6048368	9.824194952	9.45805796	10.44624
Buffer volume	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5
Enzyme volume	1.5	1.5	1.5	1.5	1.5	1.5	Enzyme volume	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5	1.5