...
Looks like only Cofilin worked. Other bands are too big and also too light. Possibly PCR did not work.
Cofilin concentration ~10ng/uL
- Picked more pENTR_hEF1a: PirB colonies, in case the one is incorrect.
...
Looks like only Cofilin worked. Other bands are too big and also too light. Possibly PCR did not work.
Cofilin concentration ~10ng/uL