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Toggle Cloak
PRACTICE #2: Co-transfection (2 fluorescent proteins under constitutive promoter)

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22 incomplete complete 7/2: Seed cells 23 incomplete 7/3: Transfect hEF1a:eYFP and hEF1a:mKate into HEK cells 24 incomplete 7/5 (__pm): Flow Cytometer

 

Y1
  • hEF1a:eYFP
(Y+R)1
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
Y2
  • hEF1a:eYFP
(Y+R)2
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
R1
  • hEF1a:mKate
(Y+R)3
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine
R2
  • hEF1a:mKate
(Y+R)4
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine

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