...
miRNA Sensors (Lab Work)
- Miniprep original EF
- PCR mRFP out of pL1F_1_S6_S7
- PCR Purify mRFP
- Run the gel for mRFP
- Pick colonies from retransformed Re Golden Gate EF and GH
- Miniprep original EF
...
- Digest mRFP cassette
- Re ligate mRFP cassette
- Transform mRFP cassette
- Retransform EF and GH
NOTES
There were no colonies to pick from EF and GH --> re transform