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  •  PCR mRFP out of pL1F_1_S6_S7
  •  PCR Purify mRFP
  •  Run the gel for mRFP
  •  Pick colonies from retransformed EF and GH
  •  Miniprep original EF
  •  Restriction digest original EF
  •  Prepare original EF for sequencing

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NOTES