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PCR of CD79B-TCS (with betaine)

Gel shows unsuccessful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  ?

Run using samples after PCR purification.

PCR of CD79B-TCS

Gel shows successful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS012 CD79B Q1_F

Reverse primer:  iGEM_AS013 CD79B QX_stop_R

Annealing temperature:  ?

Run using samples after PCR purification.

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