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tubeHyper LadderGG DONRpENTRSyk-eYFP 1pENTRSyk-eYFP 2pENTRSyk-eYFP 3pENTRSyk-eYFP 4pENTR Syk tev 1pENTR Syk tev 2pENTR Syk tev 3pENTR Syk tev 4Syk+stop 1Syk+stop 2Syk+stop 3Syk+stop 4Syk+stop 5pENTR Lyn TEV 1pENTR Lyn TEV 2pENTR Lyn TEV 3pENTR Lyn TEV 4Hyper Ladder
enzyme NsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiIPvuIIPvuIIPvuIIPvuII 
Result(P/F)  FFFFPPPFFPFFF???FFFF 
Notes               ggDONRggDONRggDONRggDONR 
Lane2122232425262728293031323334353637383940
TubeHyper LadderGG DONRpENTR Lyn-mKate 1pENTR Lyn-mKate 2pENTR Lyn-mKate 3pENTR Lyn-mKate 4pENTR Lyn-stop 1pENTR Lyn-stop 2pENTR Lyn-stop 3pENTR Lyn-stop 4CD79A-tcs-gal4 1CD79A-tcs-gal4 2CD79A-tcs-gal4 3CD79A-tcs-gal4 4CD79A-tcs-gal4 5CD79A-stop 1CD79A-stop 2CD79A-stop 3CD79A-stop 4CD79A-stop 5
Enzyme PvuIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuII
Result(P/F)  FFFFF?FFFPPFF?PFFFPF

<Gel image here>Image Added

 

Virtual gels from Geneious

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