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Cloak
22 incomplete 7/2: Seed cells 23 incomplete 7/3: Transfect hEF1a:eYFP and hEF1a:mKate into HEK cells 24 incomplete 7/5 (__pm): Flow Cytometer

 

Y1
  • _uL complete media50uL DNA-lipid complex
  • _uL hEF1a:eYFP
  • _uL Lipofectamine LTX
(Y+R)1
  • _uL complete media50uL DNA-lipid complex
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
 ////// ////// //////

-ve control

  • dummy DNA
Y2
  • _uL complete media50uL DNA-lipid complex
  • _uL hEF1a:eYFP
  • _uL Lipofectamine LTX
(Y+R)2
  • _uL complete media50uL Opti-MEM medium
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
 ////// ////// //////

-ve control

  • dummy DNA
R1
  • _uL complete media50uL DNA-lipid complex
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
(Y+R)3
  • _uL complete media50uL Opti-MEM medium
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
 ////// ////// //////

-ve/-ve control

  • cells (no action)
R2
  • _uL complete media50uL DNA-lipid complex
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
(Y+R)4
  • _uL complete media50uL Opti-MEM medium
  • _uL hEF1a:mKate
  • _uL Lipofectamine LTX
 ////// ////// //////

-ve/-ve control

  • cells (no action)

 

 

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