Page History

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Cloak

22

incomplete

7/2: Seed cells

23

incomplete

7/3: Transfect hEF1a:eYFP and hEF1a:mKate into HEK cells

24

incomplete

7/5 (__pm): Flow Cytometer

Y₁ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
Y₂ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
R₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₃ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)
R₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₄ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)

...

Cloak

14

incomplete

7/_: Seed cells

15

incomplete

7/_: Transfect TRE:mKate, hEF1a:rtTA and hEF1a:eYFP (transfection marker) into HEK cells

16

incomplete

7/_: Add DOX

18

incomplete

7/_ (__pm): Flow Cytometer

Y₁ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
Y₂ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
R₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₃ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)
R₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₄ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)

...

Cloak

29

incomplete

7/_: Seed cells

30

incomplete

7/_: Transfect hEF1a:eYFP, TRE:mKate and hEF1a:rtTA** into HEK cells

31

incomplete

7/_: Add DOX

32

incomplete

7/_ (__pm): Flow Cytometer

Y₁ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
Y₂ _uL complete media _uL hEF1a:eYFP _uL Lipofectamine LTX	(Y+R)₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve control dummy DNA
R₁ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₃ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)
R₂ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	(Y+R)₄ _uL complete media _uL hEF1a:mKate _uL Lipofectamine LTX	//////	//////	//////	-ve/-ve control cells (no action)

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