Versions Compared

Old Version 34

changes.mady.by.user Unknown User (jamesa@mit.edu)

Saved on

compared with

New Version 35

changes.mady.by.user Unknown User (aleffell@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Cloak
  • restriction-digested ten colonies' mini-prepped DNA from the plate of bacteria transformed with the eYFP-BACE1 GG product
  • restriction enzyme was BsrGI
  • note: only 8 uL of DNA was used in each digest, regardless of DNA concentration; this means that none of the restriction digests technically received enough DNA (the protocol calls for at least 500 ng, whereas most of the GG product DNA was around 20-50 ng/uL)

AL - 7/1

Toggle Cloak

Cloak

miniprepped yfp-bace1

goldengate bace1-yfp:

nbace1: .95 ul

cyfp1: 2.2

gg donr: .88

h20: 9.97

restrition digest yfp-bace1