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made kan

LC yfp-bace1

pcr n-bace1, 1 w/ dmso 1w/ betaine 1 w/ both 30 cycles @53C

JA - 7/1

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  • restriction-digested ten colonies' mini-prepped DNA from the plate of bacteria transformed with the eYFP-BACE1 GG product
  • restriction enzyme was BsrGI
  • note: only 8 uL of DNA was used in each digest, regardless of DNA concentration; this means that none of the restriction digests technically received enough DNA (the protocol calls for at least 500 ng, whereas most of the GG product DNA was around 20-50 ng/uL)