...
Ask Brian/Jeremy about changing temps/durations
Electrophoresis Gel
Prepared electrophoresis gel according to protocol
...
Lane 1: Hyperladder 1kb (5uL)
Lane 2: LacZ (mir-181c)Lane 3: LacZ (mir-144) mRFP
Include lengths of dna
When loading wells with PCR products, used Parafilm to mix 1uL of 6X Orange dye with 5uL of sample and loaded 5uL of this mixture in a lane. Ran gel at 120V for 30 minutes.
...