- PCR fusion proteins
- Golden Gate fusion proteins
- multiply fusion protein
- Put in cells and plate
- pick colonies
- miniprep and make frozen stock
- sequence
- verify sequence
- Multiply/make antibody stock
- plate stab cultures
- pick colonies
- miniprep and freeze
- sequence
- verify sequence
| | - design expression vectors
- make wiki templates
- order antibodies
- make sure we've got any
tags, dyes, etc. - anti-IgM
- anti-Syk
- anti-Lyn
- anti-primary (for blots)
- anti-pSyk (Tyr323)
- update to-do list
- Plan Gibson for BCR Plasmids
| - Place Antibody Orders (Beta Amyloid+Streptavidin)
- anti Syk
- secondary western Ig
- anti IgM
- Design Sequencing Primers
- Move Nelson's primers to freezer
- Lots of Minipreps
- Lots of Nanodropping
- (CR)
- Restriction Digests for Orange Plasmids (EE)
- Gel for 7 Plasmids (EE) (AS)
- PCR CD79B's & Gmab (AS)
- Find all blue plasmids and put in BCR Box (CR)
- Golden Gates
- LRs
|