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Cloak
id6-26
Propagation MAV1212-EBFP2 (Start 6-24)Propagation hEF1a (Start 6-25)

Culture miniprep-ed
Concentration:

Colonies picked into LB-Kan medium.
Placed into shaker at

 

 

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id6-27

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id6-27
Golden Gate Variable Expression Vector: Low Sensor (6-27)

Golden Gate miR sensing sites onto MAV1212-EBFP2, replacing LacZ.

Thermo-cycler started 2220

 

 

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id6-30

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id6-30