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(NOTE: Make sure that Buffer and Enzyme added last, enzyme after buffer)

 

Take a p20, set it to 10uL and then pipet up and down.


Example: Excel File

 

ADD
CONC.
VOLUME
ORDER
DNA1c1 ng/ulx1 = 50/c1 ul (50ng)2
............
DNAncn ng/ulxn = 50/cn ul (50ng)2
GGDonrd ng/uly = 50/d ul (100ng)2
10x T4 Ligase Buffer 2ul3
10x BSA 2ul3
BsaI (enzyme) 1ul4
T4 Ligase (enzyme) 1ul3
H20  20 - (x1 + ... + xn + y) ul1
    
TOTAL 20ul 

 Take a p20, set it to 10uL and then pipet up and down.

 

THERMOCYCLER:

(Protocol EBGG)

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