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titleJune 26

To do:

  •  Redo Syk-15aa and Lyn PCRs with DMSO
  •  Redo CD79B PCRs with GC buffer and original primers
  •  Purify and gel PCR products
  •  Run DpnI digest on TEVp product
  •  Golden Gates

Notes:

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titleJune 27
To do:
  •  Grow up colonies from Golden Gates of CD79A, CD79A-TCS-G4, Syk+stop
  •  Transform Golden Gates of Lyn+stop, Lyn-mKate, Lyn-TEVp, Syk-TEVp, Syk-eYFP
  •  PCR Gmab Heavy and Light chains