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To Do
Lab work (Can do)(Waiting on parts)(Dependent on other process)	Experiments	Planning (research?)	To Do Tomorrow
PCR fusion proteins Golden Gate fusion proteins multiply fusion protein Put in cells and plate pick colonies miniprep and make frozen stock sequence verify sequence Multiply/make antibody stock plate stab cultures pick colonies miniprep and freeze sequence verify sequence	Western blot for Syk/Lyn Check BCR localization Check IgM Binding Check Lyn Localization Check Syk Localization Characterize Syk-TEVp cleavage	design expression vectors make wiki templates finish homepage order antibodies make sure we've got any tags, dyes, etc. anti-IgM anti-Syk anti-Lyn anti-primary (for blots) anti-pSyk (Tyr323) update to-do list add daily status updates Plan Gibson for BCR Plasmids	Discuss Antibody orders with BrianPlace Antibody Orders (Beta Amyloid+Streptavidin) anti Syk secondary western Ig anti IgM Do all the PCRs!Gmab PCRs (CD79?) Design Sequencing Primers Pick Colonies for 3 GGs Plate 5 GGs Find all blue plasmids and put in BCR Box

Proteins needed

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