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We need to Gibson in eBFP2 before we can finish the sensors
siRNA MEETING WITH KYLE
mature (5' phospho) and sense (reverse complement)
copy format from 2013
send brian both sequences as a duplex (IDT has a duplex option), with all the modification
We NEED to Gibson in the eBFP2 control
each high sensor on its own transcript (not necessarily own plasmid)
QUESTIONS
what do the "r" and "m" stand for?
-rna and modification
where do the overhangs come from? why are they there?
-mature one has 2 u's (constant)