How To Please Ron/Brain
- AD blood cells from PrecisionMed? Talk to Brian?
- Meet with Kyle about designing siRNA (email sent)
- Make siRNA sequences in geneious
- Send order to Brian
- Picture of how low sensors come together
...
miRNA Sensors (Theory)
- Determine which pairs of target sites we want
- Find sequences (hopefully under 200 bp)
- Send ultramer order to Brian
- Plan new MAV vector
- Plan high sensor construction (meet with Brian)
...
miRNA Sensors (Lab Work)
- Golden Gate
- Gather miRNA target sites from Jeremy
- Nanodrop for concentrations ( ? )
- Make all 6 single low sensors (in conjunction with antibody group)
- Gather miRNA target sites from Jeremy
- Miniprep eBFP2
...
NOTES
Both LR's failed (mKate2 and L7ae)
Meeting w/ Jeremy tomorrow to talk about new MAV vector and high sensors
We need to Gibson in eBFP2 before we can finish the sensors