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PCR BACE2

  • 1 uL forward primer
  • 1 uL reverse primer
  • 1 uL template @ 1ng/uL
  • 4 uL betaine @ 5M
  • 3 uL H2O
  • 10 uL Phusion MM

30 cycles, 60 second extension time, 55C anneal temp


1 = C BACE2 w/ Betaine

2 = N BACE2 w/ Betaine

3 = C BACE2 w/ DMSO

4 = N BACE2 w/ DMSO

 

 

JA - 6/26

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BACE1 and YFP PCR labeling code:

  • A= C-YFP
  • B= N-BACE1
  • C= N-YFP
  • D= C-BACE1