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PCR of Syk-eYFP (no DMSO)

Gel shows unsuccessful PCR of Syk-eYFP from pENTR Syk (pDONR223).

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Run using samples after PCR purification.

PCR of Syk-eYFP (5% DMSO)

Gel shows successful PCR of Syk-eYFP from pENTR Syk (pDONR223).

Forward primer:  iGEM_AS004 Syk Q1_F

Reverse primer:  iGEM KRB 010 R

Annealing Temperature:  52C?

Run using samples after PCR purification.

Image Added

PCR of eYFP

Gel shows successful PCR of eYFP from pENTR_L1_EYFP_L2.

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