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Cloak

Performed miniprep as per protocol on BACE1 liquid culture

Ran PCR on eyfp and bace2, both n and c term w/ dmso @50.2C

Ran gel, 1% agarose, 120V

 

 

 

AL - 6/26

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PCR BACE2

  • 1 uL forward primer
  • 1 uL reverse primer
  • 1 uL template @ 1ng/uL
  • 4 uL betaine @ 5M
  • 3 uL H2O
  • 10 uL Phusion MM

30 cycles, 60 second extension time, 55C anneal temp