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titleJune 25

To do:

  •  Redo Syk-eYFP PCR with DMSO
  •  Image gel for yesterday's PCRs
  •  Purify and gel Syk-eYFP PCR
  •  Run remaining PCRs
  •  Purify and gel remaining PCRs
  •  Golden Gates
  •  Put gel images on parts pages
  •  Update wiki

Notes:

  • All of the original primers have arrived.
  • CD79B PCRs failed again, this time with DMSO in the mix.
  • New CD79B primers are designed and ordered.
  • When loading the gel, the Lyn wouldn't fill the wells, but instead seemed to float on top of the TAE. The reason for this is currently unknown, but it probably needs to be redone.

 

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titleJune 26

To do:

  •  Redo Syk-15aa and Lyn PCRs with DMSO
  •  Purify and gel PCR products
  •  Run DpnI digest on TEVp product
  •  Golden Gates

Notes: