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Cloak

Constructing pEXPR TRE:BACE2-eYFP and pEXPR TRE:eYFP-BACE2

  • ran PCR on BACE2 and on eYFP to produce Q1-BACE2-Q2 and Q2-eYFP-QX and Q1-eYFP-Q2 and Q2-BACE2-QX
  • order of lanes: ladder, Q2-eYFP-QX, Q1-eYFP-Q2, Q1-BACE2-Q2, Q2-BACE2-QX, (team B-cell receptor-->) 00798, FD798TOS, TCSGo14VP16

Constructing pEXPR Hef1a:BACE1-eYFP and pEXPR Hef1a:eYFP-BACE1

  • there were no colonies on the BACE1 plate, so I re-plated the BACE1 cells on a Kan plate



AL - 6/24

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- Troublshooted PCR - considered llowering annealing temp, only doing one pcr at a time

Transfered BACE1 to liquid culture

  • added 5mL LB to round bottom tube
  • added 5uL of Kan
  • picked colony
  • placed tube in shaking incubator @ 37 C