Description:
In this group of experiments, we are aiming to test whether or not our receptor proteins localize to the cell membrane. To do that, we will have HEK293 cells express LilrB2 and PirB. We will then use anti-LilrB2 and anti-PirB antibodies to test for membrane localization.
In this group of experiments, we are aiming to test the affinity of our receptors, LilrB2 and PirB to beta-amyloid oligomers. To do that, we will have HEK293 cells express LilrB2 and PirB, with and without fluorescent protein fusions. We will then apply biotinylated beta-amyloid oligomers to the LilrB2 and PirB expressing cells. To test for binding, we are going to use the biotin-binding protein streptavidin covalently bonded to a fluorophore.
Experiments Included:
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Description:Making HEK293 expressing LilrB2 and PirB on the cell membrane. Will test binding to receptors using the beta-amyloid oligomers and monomers in Experiment 4membrane localization by using anti-LilrB2 and anti-PirB antibodies in Experiment 2. Plan:
Status:
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Experiment 2: Making HEK293 cells expressing Testing membrane localization of LilrB2 and PirB fused with fluorescent protein
Experiment 3: Oligomerizing beta-amyloid from beta-amyloid monomers
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