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ProductStatusGeneStarting PlasmidStarting Plasmid ResistanceForward PrimerReverse Primer

Tm

hi/lo (anneal)

PCR's that can go together
CD79AdonecompleteCD79ApENTR CD79A (pDONR223)spectinomyciniGEM KRB 003 FiGEM KRB 004 R62/61 (61)1
CD79A-TCSdonecompleteCD79ApENTR CD79A (pDONR223)spectinomyciniGEM KRB 003 FiGEM KRB 005 R62/61 (61)1
CD79B needs gel verificationCD79BpENTR CD79B (pDONR223)spectinomyciniGEM_AS001 CD79B Q1_FiGEM_AS002 CD79B QX_stop_R72/70 (70)4
CD79B-TCS needs gel verificationCD79BpENTR CD79B (pDONR223)spectinomyciniGEM_AS001 CD79B Q1_FiGEM_AS003 CD79B Qlinker_R72/70 (70)4
TCS-Gal4VP16 needs gel verificationGal4VP16pEXPR 5 – hEF1a-FGFR-mKate-TCS(L)-G4ampicilliniGEM KRB 001 FiGEM KRB 002 R62/60 (63)6
Lyn LynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM CR 007 R61/60 (60)2
Lyn-mKate LynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM KRB 011 R61/60 (60)2
Lyn-(15aa) LynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM_AS009 Lyn Qlonglink_R61/60 (60)2
mKatedonerequires DpnI digestmKatepENTR_L1_mKate_L2kanamyciniGEM KRB 006 FiGEM KRB 007 R58/61 (58)1
Syk needs gel verificationSykpENTR Syk (pDONR223)spectinomyciniGEM_AS004 Syk Q1_FiGEM_AS005 Syk QX_stop_R54/54 (54)3
Syk-eYFP needs gel verificationSykpENTR Syk (pDONR223)spectinomyciniGEM_AS004 Syk Q1_FiGEM KRB 010 R54/54 (54)3
Syk-(15aa) SykpENTR Syk (pDONR223)spectinomyciniGEM_AS006 Syk Q1_FiGEM_AS007 Syk Qlonglink_R61/59 (59)2
eYFP needs gel verificationeYFPpENTR_L1_EYFP_L2kanamyciniGEM KRB 008 FiGEM KRB 009 R59/57 (57)5
(15aa)-TEVp TEVppENTR_L1_Tev2kanamyciniGEM_AS010 TEVp Qlonglink_FiGEM_AS011 TEVp QX_R60/60 (60)2