| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Present about cell models
miRNA Sensors (Theory)
- Prep experiments
miRNA Sensors (Lab Work)
MORNING AFTERNOON
- Gateway MAV1212-Hef1a-L7ae
- Gateway MAV1212-Hef1a-mKate2
MORNING
- Miniprep MAV1212
- Check MAV1212 colonies
- Sequence ccdb region
AFTERNOON
- PCR LacZ for low sensors
- PCR annealed oligos
- Run the gel