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PCR log page

AL - 6/12

Toggle Cloak

Cloak

added:

1uL of TRE-t (1) @ 5fM

1uL of EYFP @ 5fM

1uL of pDEST 12 @ 10fM

0.5uL of clonase

mixed by pipetting, a few air bubbles in aliquot.

left over night at room temperature

Purpose: practice

...

Cloak

Constructing pEXPR TRE:BACE2-eYFP and pEXPR TRE:eYFP-BACE2

ran PCR on BACE2 and on eYFP to give Q1-BACE2-Q

Constructing pEXPR Hef1a:BACE1-eYFP and pEXPR Hef1a:eYFP-BACE1

  • there were no colonies on the BACE1 plate, so I re-plated the BACE1 cells on a Kan plate