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Description: 

Making HEK293 expressing LilrB2 and PirB on the cell membrane. Will test binding to receptors using the beta-amyloid oligomers and monomers in Experiment 4.

Plan:

•  Order LilrB2 and PirB gBlocks 
•  Simulate Golden Gate on Geneious and make sure it works 
•  Golden Gate gBlocks into ggDONR
•  Transform bacteria with Golden Gate product and plate 
•  Pick colonies and grow in liquid culture
•  Miniprep cells 
•  LR into pDEST