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In this group of experiments, we are aiming to test the affinity of our receptors, LilrB2 and PirB to beta-amyloid oligomers. To do that, we will have HEK293 cells express LilrB2 and PirB, with and without fluorescent protein fusions. We will then apply biotinylated beta-amyloid oligomers to the LilrB2 and PirB expressing cells. To test for binding, we are going to use the biotin-binding protein streptavidin covalently bonded to a fluorophore.
Experiments Included:
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Description:Making HEK293 |
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expressing LilrB2 and PirB on |
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the cell membrane. Will test binding to receptors using the beta-amyloid oligomers and monomers in Experiment 4. Plan:
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Experiment 2: Making HEK293 cells expressing LilrB2 and PirB fused with fluorescent protein
Experiment 3: Oligomerizing beta-amyloid from beta-amyloid monomers
Experiment 4: Testing binding of monomers and oligomers to the cells from Experiment 1 and Experiment 2