...
(NOTE: Make sure that Buffer and Enzyme added last, enzyme after buffer)
| Add | Conc | Volm | Order of adding |
| DNA1 | c1 ng/ul | x1 = 50/c1 ul (50ng) | 2 |
| ... | ... | ... | ... |
| DNAn | cn ng/ul | xn = 50/cn ul (50ng) | 2 |
| GGDonr | d ng/ul | y = 50/d ul (100ng) | 2 |
| 10x T4 Ligase Buffer | 2ul | 3 | |
| BSA | 2ul | 3 | |
| BsaI (enzyme) | 1ul | 4 | |
| T4 Ligase (enzyme) | 1ul | 3 | |
| H20 | 20 - (x1 + ... + xn + y) ul | 1 | |
| Total | 20ul |
...