- re-multiply Syk+Lyn
- Nanodrop Syk+Lyn
- Sequence Syk+Lyn
- verify sequence
- PCR fusion proteins
- Golden Gate fusion proteins
- multiply fusion protein
- Put in cells and plate
- pick colonies
- miniprep and make frozen stock
- sequence
- verify sequence
- Multiply/make antibody stock
- plate stab cultures
- pick colonies
- miniprep and freeze
- sequence
- verify sequence
| | - design expression vectors
- make wiki templates
- order antibodies
- make sure we've got any
tags, dyes, etc. - anti-IgM
- anti-Syk
- anti-Lyn
- anti-antiSyk/Lyn (for blots)
- anti-pSyk (Tyr323)
- update to-do list
- design Lyn-15-TEVp
- make presentation
- Plan Gibson for BCR Plasmids
|