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For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status

designed

in planning 

in lab

ordered

in construction 

constructed 

in storage 

 

Treatment GroupReceptor Group-
NAMEGROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYOTHERNOTES
Antibody Group
pEXPR Gal4UAS:eYFPAntibodyin someone else's freezerexpression      
pEXPR Gal4UAS:mKateAntibodyin someone else's freezer

expression

      
pEXPR Tre-TEVpAntibodyin someone else's freezerexpression      
Hef1a-rtTaAntibodyin someone else's freezerexpression      
CD79A WTAntibodypossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock in -80     
CD79B WTAntibodyminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock in -80     
CD79A-TCS-Gal4VP16Antibodyplanned       
CD79B-TCS-Gal4VP16Antibodyplanned       
Lyn WTAntibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Lyn-eYFPAntibodyplanned       
Lyn point mutated (always phosphorylate)Antibodyresearching       

Syk WT

Antibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Syk-eYFP (mutant non phosphorylate)Antibodyplanned       
Syk-TEVpAntibodyplanned       
GMab kAntibodyon order       
GMab HAntibodyon order       
GMabH-TCS-Gal4VP16Antibodyplanned       
miRNA Group
hsa-miR-181cmiRNAplanned       
hsa-miR-9miRNAplanned       
hsa-miR-30d-5pmiRNAplanned       
hsa-miR-144-5pmiRNAplanned       
hsa-let-7f-5pmiRNAplanned       
has-miR-125bmiRNAplanned       
has-miR-146amiRNAplanned       
pEXPR TRE-t:BACE2-eYFPTreatmentplanned       
    • pENTR L4--TRE-t--R1
Treatmentin the lab already       
    • pENTR L1--BACE2-eYFP--L2
Treatmentplanned       
Treatmentin -80plasmid AL    
      • eYFP
Treatmentin the lab already       
    • pDEST R4--R2
Treatmentin the lab already       
pEXPR Hef1a:BACE1-eYFPTreatmentplanned       
    • pENTR L4--Hef1a--R1
Treatmentin the lab already       
    • pENTR L1--BACE1-eYFP--L2
Treatmentplanned       
      • BACE1
Treatmentto be ordered       
      • eYFP
Treatmentin the lab already       
    • pDEST R4--R2
Treatmentin the lab already       
pEXPR U6:mKate-miRNA(intronic)Treatmentplanned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)Treatmentplanned       
Experiment 1         
  • ggDONR
Receptor in storageplasmid+4 in iGEM roomLA- Kyle were not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
  • ggDONR New
Receptor in storageplasmid+4 in iGEM roomLA- KyleBrandon  ggDONR Newwill potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
  • Hef1a Entry Vector
plasmidReceptor +4 in iGEM roomlabLAplasmid Brandon   
  • Hef1a Entry Vector
 in planning - will be used to make LilrB2 expression vector
  • Destination Vector
Receptor in labplasmid      Destination Vectorin planning - will be used to make LilrB2 expression vector
  • LirB2 Expression Vector
Receptor in constructionplasmid      waiting on entry vector
    • LilrB2 Entry
    • LirB2 Expression
    • Vector
Receptor in construction - waiting on entry vectorplasmid      
    • LilrB2 Entry Vector
in construction - failing ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNAplasmid      
      • LilrB2 gBlock
Receptor used in Golden Gate - in storagegBlock-20 in iGEM roomLA- IDT  used in Golden Gate
  • PirB Expression Vector
Receptor in construction - waiting on entry vector       waiting on entry vector
    • PirB Entry Vector
Receptor in construction       - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA       
      • PirB gBlock C terminus
Receptor in used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT  used in Golden Gate
      • PirB gBlock N terminus
Receptor used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
          
          
          

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