hef1a - IgM
hef1a - IgK
hef1a - CD79A
...
| Gene | Promoter | Expression Vector | Why? |
|---|---|---|---|
| GmabH | hEF1a | ||
| GmabL | hEF1a | ||
| CD79A | hEF1a |
...
| CD79A-TCS-Gal4VP16 |
...
hef1a - CD79B
| hEF1a | |||
| CD79B | hEF1a |
| CD79B-TCS-Gal4VP16 |
hef1a - Lyn
hef1a - Syk?
TETr - Lyn-mKate
...
| hEF1a | |||
| Lyn | hEF1a | ||
| Lyn-mKate | Tet | ||
| Syk-eYFP |
...
| Tet | ||
| Syk-TEVp |
TETr - eBFP
| Tet | |||
| eBFP | Tet | Control for fluorescence | |
| mKate | GAL4/UAS | Test activation by transcription factor (Gal4VP16) | |
| rtTA | hEF1a | Required for Tet promoters |
(gal4 target) - mKate