Potential vectors to make with Gibson:
1-2 | 2-3 | 3-4 | 4-5 |
---|---|---|---|
GmabH | GmabL | CD79A | CD79B |
CD79A-TCS-Gal4VP16 | CD79B-TCS-Gal4VP16 |
Cloning Strategy for IgM heavy and light chains (outdated?)
Order G blocks "mIgM heavy chain against beta amyloid" and "mIgM kappa chain against beta amyloid" that are flanked with complementary sequences to Nelson's primer with Q1 and Qx sites
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